NGHIÊN CỨU TÁC DỤNG KHÁNG OXY HÓA INVITRO VÀ INVIVO CỦA CAO CHIẾT NẤM VÂN CHI ĐỎ (Pycnoporus sanguineus)

Abstract

Background: Pycnoporus Sanguineus is one of the 25 main medicinal mushroom species in the world with very high medicinal value, which is favored by many consumers in Asian countries, Europe, America, etc. Pycnoporus Sanguineus is known as a medicinal mushroom rich in biologically active substances such as flavonoids, polyphenols, saponins, tannins, terpenoids, coumarins, alkaloids, steroids, proanthocyanidins…. However, there have not been many studies to investigate deeply the pharmacological effects of Pycnoporus sanguineus. Objectives: 1) To investigate the invitro antioxidant effect of Pycnoporus sanguineus extract by DPPH(1,1-diphenyl-2-picrylhydrazyl) method, 2) Investigation of antioxidant effects of Pycnoporus sanguineus on a white mouse model of oxidative stress by Paraquat. Materials and methods: Extract of Pycnoporus sanguineus (MH225776); investigating the in vitro antioxidant effect of the extract by DPPH method; The effect of the extract of Pycnoporus sanguineus at doses of 500 mg/Kg and 1000 mg/Kg in  white mice model of oxidative stress with Paraquat. Results: The DPPH inhibitory activity of the Pycnoporus sanguineus extract reached the highest value (86.39%) at the concentration of 100 μg/mL. The IC50 value of the inhibitor was 55,276 μg/mL. On the other hand, the group of mice using the Pycnoporus sanguineus extract at doses of 500 mg/Kg and 1000 mg/Kg after inducing oxidative stress with Paraquat had a statistically significant (p<0.05) lower liver Malondialdehyde (MDA) content (p<0.05) compared to the group of mice using the same doses physiological saline after inducing oxidative stress with Paraquat. Conclusions: The extract of Pycnoporus sanguineus has antioxidant effects in vitro and in an experimental model of oxygen stress on white mice with Paraquat.