EVALUATION OF ANTI-INFLAMMATORY ACTIVITIES OF CLINACANTHUS NUTANS (BURM. F.) LINDAU, ACANTHACEAE BY USING IN VITRO MODELS
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Abstract
Background: Clinacanthus nutans (Burm.f.) Lindau is used worldwide for the treatment of inflammation, rheumatism, gout, and pain. Objectives: Identification anti-inflammatory effects of total extract and fractions of Clinacanthus nutans in vitro. Materials and methods: Clinacanthus nutans was harvested at Nui Cam, An Giang province. Roots, stems, and leaves were extractd by 96% ethanol. Liquid-liquid extraction of ethanol extract was performed by using dichloromethane, ethyl acetate, and water which are arranged in order of increasing polarity. Fraction extracts were employed on vacuum liquid chromatography system in order to collect more simple fractions. The evaluation of anti-inflammatory activities in vitro of extracts from different parts of Clinacanthus nutans was conducted by the inhibition of albumin denaturation and proteinase inhibitory action tests. Results: The extract from the stem of Clinacanthus nutans showed the most potent anti-inflammatory action out of the extracts from another parts. Similarly, the strongest anti-inflammatory effect was observed in ethyl acetate extract compared to extracts from other solvens. Conclusions: The results suggested that among the different parts of Clinacanthus nutans, stem possessed the most powerful anti-inflammation. This finding contributed to the use of Clinacanthus nutans properly and the discovery of chemical compounds of this medicinal plant in the future.
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Keywords
Clinacanthus nutans, anti-inflammation, inhibition of albumin denaturation, proteinase inhibitory action
References
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