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Date Published: 15/09/2023
Online Published: 15/09/2023
Abstract Views: 344
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DOI: https://doi.org/10.58490/ctump.2023i63.1253
Issue
No. 63 (2023)
Section
Article
How to Cite
Le, M. L., Vo, D. L., & Nguyen, N. N. T. (2023). DEVELOPING PROCESS FOR EVALUATING THE ENZYM α-GLUCOSIDASE INHIBITORY ACTIVITIES OF HERBAL EXTRACT USING DUAL-BEAM SPECTROPHOTOMETER. Cantho Journal of Medicine and Pharmacy, 63, 225-235. https://doi.org/10.58490/ctump.2023i63.1253

DEVELOPING PROCESS FOR EVALUATING THE ENZYM α-GLUCOSIDASE INHIBITORY ACTIVITIES OF HERBAL EXTRACT USING DUAL-BEAM SPECTROPHOTOMETER

Minh Luan Le1, Duc Linh Vo1, Ngoc Nha Thao Nguyen2,
1 Can Tho University of Medicine and Pharmacy
2 CTUMP

Main Article Content

Abstract

Background: Studies evaluating the α-glucosidase inhibitory activity of medicinal herbs often use a 96-well spectrophotometer. With a conventional 2-beam spectrophotometer, the sample processing conditions need to be developed and re-evaluated to be able to evaluate the αglucosidase inhibitory activity of herbal extracts for research purposes. Objectives: To develop a procedure and evaluate the inhibitory effect of α-glucosidase in vitro of mango leaf extract, jiaogulan leaf extract and gymnema extract on a UV-VIS dual beam spectrophotometer. Materials and methods: Mango leaf extract, jiaogulan leaf extract and gymnema extract. Evaluation of the αglucosidase inhibitory effect of the extracts by reacting with pNPG substrate consist of investigating the conditions of enzyme concentration, reaction time and influence of Na2CO3 buffer, conducting photometric measurements at 400nm wavelength using UV-VIS dual-beam spectrophotometer and applying the developed procedure to investigate the inhibitory activities of three special medicinal extracts of mango, jiaogulan and gymnema leaves. Results: The extracts of three medicinal herbs, mango, jiaogulan, gymnema and acarbose inhibited α-glucosidase with IC50 values of 39.68±0.27µg/mL, 126.94±4.58µg/mL, 92.32±0.97µg/mL and 176.09±0.26µg/mL, respectively. Conclusions: A procedure for evaluating α-glucosidase inhibitory activity in vitro on a dual-beam spectrophotometer has been developed and the results showed that the medicinal herbs of mango leaves, jiaogulan, and gymnema have the potential α-glucosiadse inhibitor.

Article Details

Keywords

Mango leaves, Jiaogulan, Gymnema, α-glucosidase, in vitro

References

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