UV-VIS SPECTROMETRY QUANTIFICATION OF TOTAL POLYPHENOLS FROM EXTRACT OF PEPEROMIA PELLUCIDA (L.) KUNTH.

Ngoc Nha Thao Nguyen1,, Thanh Long Tong1, Duy Khanh Dang1, Thi Trang Dai Nguyen1
1 Can Tho University of Medicine and Pharmacy

Main Article Content

Abstract

Background: Peperomia pellucida (L.) Kunth has polyphenol compounds, play an important role in preventing diabetes, hypertension... It is necessary to develop and validate a quantitative analysis procedure of total polyphenols in the extract of Peperomia pellucida (L.) Kunth using UV-VS method to control the quality of it. Objectives: To validate a quantitative analysis procedure of total polyphenols in the calyces of Peperomia pellucida (L.) Kunth extract using UVVS method. Material and methods: The extract of Peperomia pellucida (L.) Kunth, determining total polyphenols using UV-VIS method based on reaction between polyphenols and folin-ciocalteu reagent; validate this procedure according to ICH guidelines. Results: The quantitative analysis was performed by reaction between total polyphenols and folin-ciocalteu reagent with detective wavelength of 765nm; the method was ensured to the system suitability testing, precision, linearity, recovery, accuracy. Conclusions: The quantitative analysis procedure of total polyphenols from Peperomia pellucida (L.) Kunth extract using UV-VIS method was validated.

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References

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