STUDY ON DNA BARCODE, BOTANICAL CHARACTERISTICS, EXTRACTION AND CHARACTERIZATION OF ESSENTIAL OILS OF FOREST RED GUM - MYRTACEAE

Hong Huong Ha Ly1,, Thi Bich Ngoc Vo1, Trung Trinh Tran1, The Nhut Nguyen1, Nguyen Xuan Lam Duong2
1 Hong Bang International University
2 University of Medicine and Pharmacy at Ho Chi Minh City

Main Article Content

Abstract

Background: The leaves of Forest Red Gum were used to treat fever, headache, stomach pain, indigestion, and rheumatism in China, but there have not many scientific reports. Objectives: To investigate DNA barcodes and the botanical characteristics of Forest Red Gum; Extraction and characterization of essential oils by GC/MS. Materials and method: Forest Red Gum fresh plants were collected in Tra Vinh and then analysed the morphological and anatomical characteristics, traditional powder; the chloroplast DNA barcode on ITS1 region. Extracting essential oils. Analysising of the composition of essential oils by GC/MS. Results: Forest Red Gum was identified as Eucalypstus tereticornis J. E. SM. based on morphological characteristics and DNA barcode, and data of anatomy and traditional powder, the content of essential oils and their constituents. Conclusions: The study confirmed that the scientific name of “Forest Red Gum” in Viet Nam is Eucalypstus tereticornis J. E. SM. Myrtaceae and its essential oils components.

Article Details

References

1. Võ Văn Chi (2004), Từ điển thực vật thông dụng, Tập 1, NXB Khoa học và kĩ thuật, Hà Nội, tr.1115-1116.
2. Võ Văn Chi (2012), Từ điển cây thuốc Việt Nam, Tập 1, NXB Y học, thành phố HồChí Minh, tr. 94-95.
3. Phạm Hoàng Hộ (2000), Cây cỏ Việt Nam, Quyển II, NXB Trẻ, Thành phố Hồ Chí Minh, tr.61-62.
4. Nguyễn Tập, Ngô Văn Trại, Nguyễn Chiều và các cộng sự (2016), Danh lục cây thuốc Việt Nam, NXB Khoa học và kỹ thuật, Hà Nội, tr.42-43.
5. Cheng T., et al. (2016), “Barcoding the kingdom Plantae: new PCR primers for ITS regions of plants with improved universality and specificity”, Mol Ecol Resour, 16(1), pp.138-149.
6. Hall T. A. (1999), “BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT”, Nucleic Acid Symposium Series, 41, pp.95-98.
7. Kiran M. S., Betageri V. S., Rajith Kumar C. R., et al. (2020), “In‑vitro antibacterial, antioxidant and cytotoxic potential of silver nanoparticles synthesized using novel Eucalyptus tereticornis leaves extract”, Journal of Inorganic and Organometallic Polymers and Materials, 30(4), pp.2916-2925.
8. Sanger F., Nicklen S., Coulson A.R. (1977), “DNA sequencing with chainterminating inhibitors”, Proc Natl Acad Sci USA, 74, pp.5463-5467.
9. Sharma A. K., Dutt D., Upadhyaya J. S., Roy T. K. (2011), “Anatomical, morphological, and chemical characterization of bambusa tulda, dendrocalamus hamiltonii, bambusa balcooa, malocana baccifera, bambusa arundinacea and eucalyptus tereticornis”, Bambusa spp. & papermaking, BioResources, 6(4), pp.5062-5073.
10.Sreevani P., Rao R.V. (2014), “Variation in basic Density and Anatomical properties of Eucalyptus tereticornis Sm. Clones”, International Science Congress Association, 3(2), pp.271-274.