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Date Published: 24/10/2023
Online Published: 24/10/2023
Abstract Views: 211
Views PDF: 131
Issue
No. 43 (2021)
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Article
How to Cite
Huynh, H. A. T., Pham, D. V., & Do, C. M. V. T. (2023). SIMULTANEOUS DETERMINATION OF FLUOROQUINOLON IN SHRIMP BY LC-MS/MS. Cantho Journal of Medicine and Pharmacy, 43, 45-52. https://tapchi.ctump.edu.vn/index.php/ctump/article/view/1078

SIMULTANEOUS DETERMINATION OF FLUOROQUINOLON IN SHRIMP BY LC-MS/MS

Huynh Anh Thi Huynh1, Doan Vi Pham2, Chau Minh Vinh Tho Do1,
1 Can Tho University of Medicine and Pharmacy
2 Tay Do University

Main Article Content

Abstract

  Background: The overuse and the lack of the management of antibiotic can lead to the persistence of their residues on aquaculture products. Objectives: To develop, validate a procedure for simultaneous quantitative analaysis of fluoroquinolon in shrimp samples by LC-MS/MS according to AOAC, EC guidelines; procedure application for shrimp samples in Kien Giang province. Materials and methods: Enrofloxacin, ciprofloxacin, sarafloxacin, flumequin and shrimp samples collected in Kien Giang province, develop simultaneous determination procedure by LCMS/MS system. Results: The suitable mass spectrometry parameters were used to quantify the fragment ion of sarafloxacin (386.02→342), ciprofoxacin (332.03→288), enrofloxacin (360.06→316), flumequin (261.98→244). Suitable chromatographic parameters: GL InertSustain C18 (4.6 × 250 mm, 5 µm), gradient elution program. The method was validated with a wide linear range with correlation coefficient R2>0.99, good accuracy (87.6–100.06%) and inter-day precision (RSD<7.5%), LOD and LOQ values were 0.03-0.5ppb and 0.125-1.5ppb, respectively. The antibiotic residues of 10 shrimp samples were not exceeded the LOD of method. Conclusion: Developed the analytical procedure for 4 fluoroquinolon antibiotics in shrimp by LC-MS/MS, the analytical procedure was validated according to EC 657/2002 guidline, the analysis results on 10 shrimp samples collected in Kien Giang did not detect residues of these 4 antibiotics. 

Article Details

Keywords

Fluoroquinolon, enrofloxacin, ciprofloxacin, sarafloxacin, flumequin, LC-MS/MS, shrimp

References

1. Trần Thanh Trúc, Trần Thị Như Trang (2013), “Phân tích đồng thời các kháng sinh quinolone trong thịt, tôm, cá bằng phương pháp sắc kí lỏng ghép khối phổ”, Tạp chí Phát triển KH&CN, 16(2), tr.39-46.
2. AOAC International (2002), “AOAC Guidelines for Single Laborator Validation of Chemical Methods for Dietary Supplements and Botanicals”, Section 3.3, 3.4.1, 3.4.2, 3.4.6, pp.18-22.
3. Bortolotte, A. R., Daniel, D., de Campos Braga, P. A., & Reyes, F. G. R. (2019). A simple and high-throughput method for multiresidue and multiclass quantitation of antimicrobials in pangasius (Pangasionodon hypophthalmus) fillet by liquid chromatography coupled with tandem mass spectrometry. Journal of Chromatography B, 1124, 17-25.
4. Braun, G., Braun, M., Kruse, J., Amelung, W., Renaud, F. G., Khoi, C. M., Sebesvari, Z. (2019). Pesticides and antibiotics in permanent rice, alternating rice-shrimp and permanent shrimp systems of the coastal Mekong Delta, Vietnam. Environment international, 127, 442-451.
5. European Union (2002), Commission decision of 12 August 2002 implementing Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results.
6. Lu, Z., Deng, F., He, R., Tan, L., Luo, X., Pan, X., & Yang, Z. (2019). A pass-through solidphase extraction clean-up method for the determination of 11 quinolone antibiotics in chicken meat and egg samples using ultra-performance liquid chromatography tandem mass spectrometry. Microchemical Journal, 151, 104213.
7. Saha, S., Singh, A. K., Keshari, A. K., Raj, V., Rai, A., & Maity, S. (2018). Modern extraction techniques for drugs and medicinal agents. Ingredients extraction by physicochemical methods in food, pp.65-106.
8. Soumya, R., & Sachindra, N. M. (2015). Carotenoids from fishery resources. Fish processing byproducts: quality assessment and applications. Studium Press, Houston, 273-298.
9. Ström, G. H., Björklund, H., Barnes, A. C., Da, C. T., Nhi, N. H. Y., Lan, T. T., Boqvist, S. (2019). Antibiotic Use by Small Scale Farmers for Freshwater Aquaculture in the Upper Mekong Delta, Vietnam. Journal of aquatic animal health, 31(3), 290-298.
11. Priyanka, V. J. J., Chauhan, S. L., & Garg, S. R. (2019). Analysis of Quinolones Residues in Milk using High Performance Liquid Chromatography. Int. J. Curr. Microbiol. App. Sci, 8(2), 3049-3058.
12. Saxena, S. K., Rangasamy, R., Krishnan, A. A., Singh, D. P., Uke, S. P., Malekadi, P. K., & Gupta, A. (2018). Simultaneous determination of multi-residue and multi-class antibiotics in aquaculture shrimps by UPLC-MS/MS. Food chemistry, 260, 336-343.