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SIMULTANEOUS DETERMINATION OF SULFAQUINOXALINE, SULFAMETHOXAZOLE, SULFATHIAZOLE AND TRIMETHOPRIM ANTIBIOTIC RESIDUES FREQUENTLY USED IN POULTRY MEAT BY LC-MS/MS
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Abstract
Background: The control of antibiotics that are being abused in agriculture, especially in poultry, is essential. Objectives: To develop and validate a procedure for simultaneous quantitative analaysis of sulfaquinoxaline, sulfamethoxazole, sulfathiazole, trimethoprim in chicken samples by LC-MS/MS according to AOAC, EC guidelines; procedure application for chicken samples in Kien Giang province. Materials and methods: Sulfaquinoxaline, sulfamethoxazole, sulfathiazole, trimethoprim standard substances and chicken samples collected in Kien Giang province, develop determination procedure using LC-MS/MS method. Results: The suitable mass spectrometry parameters to quantify the parent/fragment ion of sulfaquinoxaline (301/92), sulfamethoxazole (254/92), sulfathiazole (256/156), trimethoprim (291/123). Suitable chromatographic parameters: Poroshell 120 Phenyl-Hexyl column (4.6x150 mm, 2.7 µm), gradient elution program, sample injection volume and time of 10 µL and 13 minutes, respectively. The method was validated with a good accuracy (87.9–102.3%) and precision (RSD < 8.5%), the linear range of 5-200 ppb and 2.5– 100 ppb (r2>0.99), LOD and LOQ values were 0.0625-0.125 ppb and 0.125-0.25 ppb for sulfonamides and trimethoprim, respectively. The application results on 9 chicken samples have not detect antibiotic residues. Conclusion: Developed a selective, accurate, and highly reliable analytical procedure for the simultaneous determination of 4 antibiotics sulfaquinoxaline, sulfamethoxazole, sulfathiazole, trimethoprim on poultry meat samples with satisfactory validation results, the application results on market samples have not detected antibiotic residues.
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Keywords
Sulfonamid, trimethoprim, LC-MS/MS, poultry
References
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