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Date Published: 12/06/2023
Online Published: 12/06/2023
Abstract Views: 634
Views PDF: 1678
Issue
No. 45 (2022)
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Article
How to Cite
Vu, D. T., Le, T. H. M., & Truong, C. K. (2023). HbA1c MEASUREMENT METHODS: A REVIEW. Cantho Journal of Medicine and Pharmacy, 45, 222-232. https://tapchi.ctump.edu.vn/index.php/ctump/article/view/1015

HbA1c MEASUREMENT METHODS: A REVIEW

Dinh Trung Vu1,, Thi Hoang My Le2, Cong Khanh Truong1
1 Kien Giang Traditional Medicine Hospital
2 Can Tho University of Medicine and Pharmacy

Main Article Content

Abstract

Hemoglobin A1c (HbA1c) is an important laboratory parameter to screen, diagnose and monitor the results of diabetes mellitus treatment, which is a metabolic disorder. In 1968, the first HbA1c was identified. Then, there have been many studies proving correlation between HbA1c and venous blood glucose. In addition, there have also been many new approaches to HbA1c measurement. Currently, there are five methods to HbA1c assay, the test results are standardized to the International Federation of Clinical Chemistry (IFCC) Reference Measurement Procedure (RMP) in harmony with the efforts of the National Glycohemoglobin Standardization Program (NGSP): Immuno assays, enzymatic assays, boronat affinity chromatography, ion exchange high pressure liquid chromatography, capillary electrophoresis. This review article describes the principles of five methods for HbA1c measurement, their advantages and disadvantages, and their development in future. Based on published scientific data, the results showed that good agreement between methods for HbA1c measurement in blood samples with non-hemoglobinopathy and these methods were affected by blood samples with hemoglobinopathy. This was not only a stimulus for the improvement of HbA1c measurement technology but also a reference for research in future.

Article Details

Keywords

Hemoglobin A1c, capillary electrophoresis, HPLC, immunoassay, affinity chromatography, enzymatic assay, methods for HbA1c measurement

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