ASSESSMENT THE GROWTH INHIBITION EFFECT OF LIVER CANCER CELLS OF CAPSULES CONTAIN GRAVIOLA EXTRACT (ANNONA MURICATA (L.) ANNONACEAE) ON IN VITRO AND IN VIVO MODELS
Main Article Content
Abstract
Backgrounds: Annona muricata (soursop or graviola) of Annonaceae family has been used worldwide as a natural medicinal for many years, and it has been reported to have a broad range of therapeutic effects. Most of the research on graviola focuses on compounds called acetogenins (ACGs). The separate research groups have confirmed that these chemicals have significant antitumorous properties and selective toxicity against various types of cancer cells (without harming healthy cells). Objectives: The aim of this study was to assess ability to inhibit the growth of liver cancer cells of capsule contain graviola extracts on in vitro and in vivo. Materials and methods: Estimate cytotoxic activity of three types of extracts were extracted from three different polarization solvents by MTT [(3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromid)] method on HepG2 liver cancer cells; Modeling of liver tumor in immunocompromised male mice and preliminary investigation of the inhibitory effect of capsules. Results: Identify IC50 values of ethanol extract, ethyl acetate extract and n-hexane extract respectively: 25.73 µg/mL, 39.40 µg/mL, 61.01 µg/mL and the effect was assessed on in vivo model. Conclusion: The capsules contain graviola extracts are capable of inhibiting the growth of human hepatocellular carcinoma HepG2 and on a liver tumor model of immunocompromised male mice.
Keywords
graviola, acetogenins, HepG2
Article Details
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