Thanh bên bài viết
Số lượt xem PDF: 68
EVALUATING THE MORPHOLOGICAL CHARACTERISTICS AND VISCERAL FAT OF MALE MICE GENERATED OBESITY MODEL BY A HIGH-FAT DIET
Nội dung chính của bài viết
Tóm tắt
Background: Obesity is a global health issue caused by consuming too much energy compared to the body's needs, which negatively impacts almost every organ of the body. As a result, generating an experimental obesity model is critical for researching new drugs to improve obesity and its consequences. Objectives: Generating obesity model of male mice over a 6-week period of feeding by a high-fat diet. Materials and methods: In this experimental research, 24 male mice (Swiss albino) of 3,5 weeks old were divided into 2 groups, each containing 12 mice: control group fed by a normal-fat diet (NFD) and obesity group fed by a high-fat diet (HFD) in a period of 6 weeks. Evaluation was conducted at the beginning of research, every 7 days and at the end of the study. Results: The mean of body weight, weight gain, body length, body mass index (BMI), chest and waist circumference of HFD group had a statistically significant difference from the end of week 2. The morphological difference was more remarkable at week 3 (p<0.001). After 6 weeks of study, the weight gain rate of HFD group was 131.32% higher than the NFD group. It was also noticeable that the weight of omental fat and renal fat (both sides) in the HFD group was significantly higher than that of the NFD group (p<0,001). Conclusions: Research has successfully generated an obesity model in male mice after a period of 6 weeks by a HFD (640Kcal/100g, 52-53% lipid).
Chi tiết bài viết
Từ khóa
Swiss albino, male mice, obesity, high-fat diet
Tài liệu tham khảo
2. Lam Quang Duc, Nguyen Viet Dien, Tran Ngoc Minh et al. (2019), “Evaluation of the effect of film-coated tablet containing azadirachta indica leaf extract on stabilizing blood sugar level in obese mice developed hyperglycemia induced by oral glucose tolerance test”, Can Tho Journal of Medicine and Pharmacy, No. 22-23-24-25, pp. 1-6.
3. Mehrdad Ghorbanlou et al. (2020), “Possible ameliorating effects of Glycyrrhiza Glabra (Licorice) on the sperm parameters in rats under high fat diet”, Endocrine Regulations, Vol. 54, No. 1, pp. 22-30.
4. Ngo Thi Quynh and Do Ngoc Lien (2012), “Study on the effect of lowering blood sugar and blood fat of “San thuyen” extract (Syzygium polyanthum (Wight) Wamp)”, Thesis statement for the degree of Master of Experimental Biology, University of Sciences.
5. Nguyen Cao Tri, Vo Minh Tuan, Ngo My Tien et al. (2019), “Generating and evaluating high fat diet - induced obesity Swiss albino mice model”, Journal of Science and Technology of Industrial University of Ho Chi Minh City, No. 39B, pp. 181-190.
6. Nguyen Thi Hoa et al. (2020), “Obesity rat model by high-fat diet”, Vietnam Medical Journal, Vol. 493 (1), pp. 7-13.
7. Nicole O. Palmer et al. (2012), “Impact of obesity on male fertility, sperm function and molecular composition”, Spermatogenesis, Vol. 2, No. 4, pp. 1-11.
8. Tran Thi Minh, Nguyen Ngoc Tanh, Pham Thanh Truc Loan et al. (2021), “Ability to reduce dyslipidemia syndrome of Gonaderma lucidum”, Scientific Journal of Van Lang University, No. 27, pp. 82-87.
9. Tran Trung Kien (2010), “Study on the anti-obesity and hypoglycemic effects of some biologically active natural substances in sweet potato (Ipomoea batatas (L.) Lam) in a mouse model of obesity and type 2 diabetes”, Science and Technology Journal, Hung Vuong University, No. 3(16), pp. 11-14.