GENOTYPE AND PHENOTYPE IDENTIFICATION OF METHICILLINRESISTANT STAPHYLOCOCCUS AUREUS: NOVEL DISCOVERIES FROM PNEUMONIA CASE SERIES
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Tóm tắt
Background: Staphylococcus spp. identification results showed a difference between phenotypic and genotypic methods. Objectives: To observe the phenotype and genotype of MRSA in patients with pneumonia. Materials and methods: We reviewed 10 patients with methicillinresistant Staphylococcus aureus (MRSA) isolated in lower respiratory tract samples. Samples were investigated with the VITEK system and real-time PCR methods with the MRSA Quant Real-TM kit (Sacace™ Biotechnologies, Italy). Results: The results recognized 10 cases of MRSA infections from culture. Still, from these samples, real-time PCR detected 4 MRSA, 5 MRCoNS (methicillin-resistant Coagulase-negative Staphylococci), 1 MRCoNS or MRCoNS, and MRSA. Interestingly, levels of MICs were different between the MRSA and MRCoNS genotypes. Conclusion: Our results showed that the MRSA phenotype method was incompatible with the genotype method. The existence of MRCoNS in these cases can be evidence that MRCoNS in lower respiratory tract samples should also be considered causative agents in pneumonia.
Từ khóa
Methicillin- Staphylococcus aureus, Coagulase-negative Staphylococci, real-time PCR
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