OPTIMIZATION OF THE EXTRACTION PROCESS OF POLYPHENOLS FROM EHRETIA ASPERULA LEAVES IN CAN THO
Nội dung chính của bài viết
Tóm tắt
Background: Ehretia asperula is a medicinal plant rich in polyphenols, which have significant antioxidant properties. Optimizing the extraction process is essential to enhance polyphenols yield and maximize its potential applications in pharmaceuticals. Objectives: To optimize the extraction conditions of polyphenols from Ehretia asperula leaves using the BoxBehnken design. Materials and methods: The optimization was performed using Design Expert 13.0, evaluating three independent variables: temperature (60°C, 65°C, 70°C), solvent-to-material ratio (1/30, 1/35, 1/40), and ethanol concentration (60%, 70%, 80%). Total polyphenol content was determined using the Folin-Ciocalteu method. Results: The optimal extraction conditions were identified as an ethanol concentration of 67.28%, a temperature of 68.91°C, and a solvent-tomaterial ratio of 1/35.1557 (w/v), yielding 96.442 mg GAE/g of extract. Experimental validation showed a polyphenol content of 89.593 mg GAE/g, confirming the model's reliability. Additionally, the study determined that five extraction cycles were necessary to achieve maximum polyphenols recovery. Conclusions: The optimized extraction process significantly enhances the yield of polyphenols from Ehretia asperula, providing a reliable method for future pharmaceutical and nutraceutical applications.
Từ khóa
Ehretia asperula, polyphenols extraction, optimization, Box-Behnken design
Chi tiết bài viết
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